Generator

Part:BBa_K3052015:Design

Designed by: Ma WenHe   Group: iGEM19_XJTU-CHINA   (2019-10-13)


Ptrc+linalool synthase(DHJ)+bacterial terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 577
    Illegal PstI site found at 658
    Illegal PstI site found at 1081
    Illegal PstI site found at 1147
    Illegal PstI site found at 1237
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 577
    Illegal PstI site found at 658
    Illegal PstI site found at 1081
    Illegal PstI site found at 1147
    Illegal PstI site found at 1237
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 577
    Illegal PstI site found at 658
    Illegal PstI site found at 1081
    Illegal PstI site found at 1147
    Illegal PstI site found at 1237
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 577
    Illegal PstI site found at 658
    Illegal PstI site found at 1081
    Illegal PstI site found at 1147
    Illegal PstI site found at 1237
    Illegal NgoMIV site found at 108
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part does not contain lacI so it cannot be directly induced by IPTG. In our design, we transfer this circuit with another circuit producing GPP which contains lacI to make it inducible by IPTG.


Source

This part is acquired from chemical synthesis.


References